Equal-frequency motion control for volume optimization ofnucleic acid extraction samples
JIANG Xin, YAO Jia, LI Dongshu, WANG Tianyi, LI Jinze, LI Yingxue, ZHOU Lianqun
1. Xuzhou Medical University, Xuzhou 221000, China;
2. Key Laboratory of Biomedical Laboratory Technology, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China;
3. Suzhou Guoke Xingan Medical Technology Company, Suzhou 215163, China
Abstract: In order to optimize the sample volume of nucleic acid extraction under oscillating motion, the equal frequency motion control algorithm is applied to the nucleic acid extraction process. By improving the stability of the nucleic acid extraction process, the sample volume of nucleic acid under oscillating motion is effectively improved. Based on the STM32 high precision control platform, the step motor was controlled by equal frequency discretization. The Sigmoid curve was used to realize the acceleration and deceleration process, and the smooth and accurate control of the step motor was realized. The proposed constant frequency discrete acceleration and deceleration motion control algorithm solved the problem of unstable speed change in the process of variable speed. Compared with equal-time sampling, the experimental results show that under the same experimental parameters, the method of equal-frequency discretization can achieve the target frequency faster than that of equal-time discretization. Compared with the equal time discretization method, the equal frequency discretization method improves the positioning accuracy by 39.47%. In the application of nucleic acid extraction, the constant frequency dispersion method can effectively improve the nucleic acid sample volume by 20.62 μL compared with the constant time dispersion method. Compared with the traditional Sigmoid function model to realize the S-curve acceleration and deceleration control algorithm, the equal frequency discrete method can improve the positioning accuracy of motion and make the extraction process smoother, and effectively improve the sample volume under the oscillating motion of nucleic acid extraction.
Keywords: stepper motor;nucleic acid extraction;acceleration and deceleration control;Sigmoid function;positioning accuracy
2023, 49(11):157-165  收稿日期: 2023-04-07;收到修改稿日期: 2023-05-20
基金项目: 国家自然科学基金青年基金资助项目（61901469）；江苏省重点研究开发项目（BE2020768）；中国科学院科研仪器设备研制项目(YJKYYQ20200046)
作者简介: 蒋新(1998-),女,湖南益阳市人,硕士研究生,专业方向为生物医学测量技术。
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