Development of avian leukosis virus p27 protein lyopgilized powder reference material
ZHAO Xu, SHI Lianhua, WU Liqing, JIN Youxun
National Institute of Metrology, China, Beijing 100029, China
Abstract: In order to meet the requirements of traceability of clinical p27 protein measurement results, reference materials of avian leukosis virus p27 protein lyophilized powder were developed. The value of the reference materials of avian leukosis virus p27 protein lyophilized powder were evaluated by HPLC-IDMS/MS in several laboratories, and the certified values were (1.46 ± 0.13) mg/mL. The developed method is accurate and reliable, it is able to be applied for the development of p27 protein reference material. The result indicated that the reference materials were sufficient homogeneity and had good stability, could be stored for up to 7 days at –20 ℃, 4 ℃ and room temperature. It could be stored for 6 months at –70 ℃. The development of p27 protein reference material is important for quality control of avian leukemia assays and for the evaluation of different avian leukemia assays.
Keywords: avian leukosis;p27 protein;isotope dilution mass spectrometry;reference material
2023, 49(11):166-175  收稿日期: 2023-03-07;收到修改稿日期: 2023-04-16
基金项目: 国家重点研发计划项目（2022YFC2009600，2022YFC2009602）
作者简介: 赵旭(1997-),男,新疆额敏县人,硕士研究生,专业方向为蛋白质测量技术与计量。
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